Dualex植物葉綠素儀應(yīng)用：葉片不同發(fā)育階段葉綠素酶的定位

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

摘要 

背景：葉綠素酶（Chlases）是植物細(xì)胞中的管家蛋白。脫植酸酶可以催化葉綠素（Chl）形成葉綠素內(nèi)酯，但葉綠素酶在植物細(xì)胞中的分布仍然具有爭論。本研究制備了PmCLH2抗體，并用免疫金標(biāo)記技術(shù)檢測了大果委陵菜（Pachira macrocarpa，Pm）幼葉、成熟葉、黃化葉和衰老葉四個(gè)發(fā)育階段的葉綠素酶定位。結(jié)果：透射電鏡觀察結(jié)果表明，在葉綠體的幼齡期、成熟期和變黃期，葉綠體的膜、基粒、葉綠體的類囊體膜、細(xì)胞質(zhì)和液泡等部分全面發(fā)現(xiàn)了葉綠素，但在細(xì)胞壁、質(zhì)膜、線粒體和細(xì)胞核中沒有發(fā)現(xiàn)。結(jié)論：在衰老期，PmChlases主要存在于液泡中，但在葉綠體中很少發(fā)現(xiàn)。提出了一條解釋高等植物中葉綠素、葉綠素酶和葉綠體的產(chǎn)生和死亡的途徑。

Immunogold-labelling localization of chlorophyllase at different developmental stages of Pachira macrocarpa leaves 

Abstract

Background: Chlorophyllases (Chlases) are housekeeping proteins in plant cells. The dephytylating enzymes can catalyze chlorophyll (Chl) to form chlorophyllide, but the distribution of Chlases in plant cells is still an interesting debate. In this study, antibody of PmCLH2 was made and used by immunogold-labelling technique to detect the location of Chlase of Pachira macrocarpa (Pm) leaves at four developmental stages, including young, mature, yellowing, and senesced stages. Results: The transmission electron microscopy results show that Chlases were comprehensively found in portions of chloroplast, such as the inner membrane of the envelope, grana, and the thylakoid membrane of the chloroplast, cytosol, and vacuoles at young, mature, and yellowing stages of Pm leaves, but not in the cell wall, plasma membrane, mitochondria, and nucleus. Conclusions: PmChlases were mainly detected in vacuoles at the senescent stage, but a few were found in the chloroplasts. A pathway is proposed to explain the birth and death of Chl, Chlase, and chloroplasts in higher plants.

相關(guān)閱讀

Dualex 便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

PESSL便攜式便攜式植物多酚-葉綠素儀原理

Dualex植物多酚-葉綠素儀：腐殖酸通過鐵依賴和非鐵依賴的協(xié)同機(jī)制緩解禾本科植物的鐵失綠癥

Dualex植物多酚-葉綠素儀：硝化抑制劑和/或間作效應(yīng)下玉米（和小麥灌溉種植系統(tǒng)中的硝酸鹽淋失

Dualex植物多酚-葉綠素儀：與保持綠色表達(dá)相關(guān)的葉片和冠層性狀與對水分脅迫具有不同耐性的小麥基因型的產(chǎn)量構(gòu)成密切相關(guān)

Dualex植物多酚-葉綠素儀：基于地面和智能傳感器的冬小麥營養(yǎng)營養(yǎng)儀

Dualex植物多酚-葉綠素儀：增加角質(zhì)層蠟沉積不會改變殘余葉片蒸騰作用

Dualex植物多酚-葉綠素儀--氮平衡指數(shù)：不同生物刺激劑對水培生菜的效果比較

Dualex植物多酚-葉綠素儀：固體消化物和葡萄園冬季修剪堆肥替代苗圃中微繁殖高叢藍(lán)莓生長基質(zhì)中的泥炭研究

Dualex植物多酚-葉綠素儀：不同灌溉制度下根際細(xì)菌和藻類對金盞草生理生化指標(biāo)的影響

Dualex植物多酚-葉綠素儀：Sentinel-2A與近端傳感器數(shù)據(jù)協(xié)同提取水稻不同生育期生化參數(shù)

Dualex植物多酚-葉綠素儀：三種便攜式光學(xué)傳感器在冬小麥氮素狀況無損診斷中的應(yīng)用